Biotransformasi Patchouli Alkohol Dalam Minyak Nilam Menggunakan Isolat Bakteri Dari Limbah Pome (Palm Oil Mill Effluent)

Mainisa, Suti (2020) Biotransformasi Patchouli Alkohol Dalam Minyak Nilam Menggunakan Isolat Bakteri Dari Limbah Pome (Palm Oil Mill Effluent). Diploma thesis, Universitas Andalas.

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Abstract

Patchouli oil is an essential oil that is widely used in the perfume and cosmetic industries, most of the oil consist of patchouli alcohol. Patchouli alcohol biotransformation using acetic acid with the lipase enzyme as a catalyst is carried out to produce its derivative compounds, one of which is the ester compound (patchouli acetate) which has a more specific odor (soft woody) trough the esterification process. In this study, the use of enzymes as biocatalysts is intended to replace the role of acids as a more environmentally friendly alternative. The source of the enzyme used comes from bacterial cell culture isolated from POME (Palm Oil Mill Eflluent) and has been selected for survival against patchouli oil which is identified as a group of Bacillus sp and Corynebacterium sp. This study aims to look at the potential of bacterial isolates obtained in the patchouli alcohol biotransformation process and any other byproducts formed from this process. The components of the patchouili oil compiler based on GC-MS analysis are composed of 16 compound components where the largest component is found at a retention time of 7.957 minutes with an area of 88.69% identified as patchouli alcohol. Biotransformation on a sterile Nutrient Broth medium for 24 hours, 150 rpm was analyzed qualitatively using a uv spectrophotometer by comparing the spectrum of compounds resulting from biotransformation with patchouli oil standards. The results of the analysis showed that the formation of new compounds was marked by differences in the spectrum of compounds with standards in products using bacterial isolates of bacillus sp. Furthermore, it was comfirmed quantitatively using GC-MS where the results i=of the analysis showed that there were 24 compound components with the largest percentage, namely delta-guaiene (29.95%), alpha-guaiene (22.21%), Seychellene (12.83%), alpha-patcholene (7.95%) and beta-patchoulene (6.71%) where the patchouli alcohol compound no longer exists and the desired ester derivative compound is not formed. It can be concluded that bacterial isolates from POME have no potential in the process of biotransformation of patchouli alcohol into their ester derrivates.

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