PERBANDINGAN PROFIL MIKROBIOTA VAGINA PADA PASIEN ENDOMETRIOSIS DAN TANPA ENDOMETRIOSIS

Meria, Sef Zani (2024) PERBANDINGAN PROFIL MIKROBIOTA VAGINA PADA PASIEN ENDOMETRIOSIS DAN TANPA ENDOMETRIOSIS. Spesialis thesis, Universitas Andalas.

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Abstract

Introduction: Endometriosis is a complex and heterogeneous gynecological disease that affect approximately 10% of women in reproductive age, involving inflammation and immune regulation. The vaginal microbiota with its diversity is an integral part of vaginal microecology that interacts with vaginal anatomy, the endocrine system and local vaginal immunity. The disruption in microecology, known as dysbiosis is known to trigger a variety of inflammatory diseases. The relationship between vaginal dysbiosis and endometriosis remains controversial Objective: To determine the comparison of vaginal microbiota profiles in patients with endometriosis and without endometriosis Method: The study was using the case control design on patients diagnosed with endometriosis at Dr. M. Djamil Hospital. The study was conducted on December 2023 to June 2024 at the Polyclinic of Dr. M. Djamil Hospital and the Center for Infectious Disease Diagnostic and Research Laboratory. The diagnosis of endometriosis was confirmed via ultrasonography and histology. Microbiota were measured with miSeq Illumina Next Generation Sequencing. Comparison of microbiota profiles was carried out using the Mann Whitney test. Comparison of dysbiosis was carried out using the Chi square test. Results: There were 40 respondents divided into endometriosis group (n=20) and without endometriosis group (n=20). At the phylum level, the vaginal microbiota profile in endometriosis group were dominated with Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes, while in without endometriosis group consist of Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria. At the genus level, endometriosis group were dominated with Lactobacillus sp., Desulfovibrio sp., Flavobacterium sp., and Gardnerella sp., while in without endometriosis group were Lactobacillus sp., Prevotella sp., Desulfovibrio sp., and Bifidobacterium sp. There was no difference in the abundance of microbiota (p>0.05) or the incidence of dysbiosis (p>0.05) between endometriosis group and without endometriosis group at the genus and phylum levels. Conclusion: There was no difference in the abundance of microbiota between endometriosis group and without endometriosis group. However, there were differences in the order of dominance of the vaginal microbiota after Firmicutes at the phylum level and Lactobacillus at the genus level between both groups. Keywords: Endometriosis, dysbiosis, vaginal microbiota, Lactobacillus, Flavobacterium, Firmicutes, Proteobacteria

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