ISOLATION, POTENCY AND CHARACTERIZATION OF BREAST MILK BACTERIA AS A PROBIOTIC CANDIDATE

Fitra, Al Zakia Asyari (2019) ISOLATION, POTENCY AND CHARACTERIZATION OF BREAST MILK BACTERIA AS A PROBIOTIC CANDIDATE. Diploma thesis, Universitas Andalas.

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Abstract

Natural bacteria from three sample breast milk with several ages infant 0 – 2 month, 2 – 4 month and 4 – 6 month have been analyzed and compared. This study aims to analyze the composition of microflora and proteolytic bacteria in breast milk, to compare the character of potential acid fermentation isolates as probiotic candidates. The study was conducted with survey methods and data were analyzed descriptively. The results of this study include, the proportional presence of fermentative bacteria in three highest different samples obtained at the several age potential isolates of fresh breast milk, including Lactobacillus (Lactobacillus sp. 1 (ASI A1, ASI B1, ASI C1), Lactobacillus sp2. (ASI B2, ASI C2) and Lactobacillus sp3. (ASI A2) and the highest Fermentative Index found in ASI B2 isolates, and highest Proteolytic Index found also in ASI B2 isolates. Hemolysis Tests of the six isolates (ASI A1, ASI A2, ASI B1, ASI B2, ASI C1, ASI C2) were not pathogenic (negative pathogens) which were marked d with no formation of clear zone regions (zone of hemolysis) around isolates, and ASI A1, ASI A2, ASI B1, ASI B2, ASI C1, ASI C2 including the class of lactic acid bacteria.

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