EFEK PEMBERIAN LACTOCOCCUS LACTIS D4 TERHADAP GAMBARAN HISTOPATOLOGI FIBROSIS GINJAL PADA TIKUS MODEL UNILATERAL URETERAL OBSTRUCTION

Edo, Bagus Tantyo (2024) EFEK PEMBERIAN LACTOCOCCUS LACTIS D4 TERHADAP GAMBARAN HISTOPATOLOGI FIBROSIS GINJAL PADA TIKUS MODEL UNILATERAL URETERAL OBSTRUCTION. Spesialis thesis, UNIVERSITAS ANDALAS.

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Abstract

Background : Obstructive nephropathy is a common cause of chronic kidney disease (CKD), characterized by renal fibrosis due to urinary flow obstruction. Lactococcus lactis D4, a probiotic strain isolated from dadiah (a traditional fermented buffalo milk from Indonesia), has demonstrated potential antiinflammatory and immunomodulatory effects, suggesting a therapeutic role in reducing renal fibrosis. Objective : This study aims to evaluate the effect of Lactococcus lactis D4 on renal fibrosis in a rat model of unilateral ureteral obstruction (UUO) using histopathological analysis with Sirius red staining. Methods : A laboratory-based experimental study was conducted on male SpragueDawley rats aged 6-7 weeks. The rats were divided into three groups: Sham (laparotomy without UUO), P1 (UUO without Lactococcus lactis D4), and P2 (UUO with Lactococcus lactis D4). Lactococcus lactis D4 was administered orally at a dose of 8x109 CFU/mL for 7 days. Histological analysis of renal fibrosis was performed using Sirius red staining, and quantitative assessment was done using ImageJ software. Data were analyzed statistically using ANOVA followed by post- hoc testing. Results : The Sham group showed minimal fibrosis (17.07 ± 0.58%), while the P1 group experienced a significant increase in fibrosis (28.68 ± 3.27%, p = 0.013). The P2 group, which received Lactococcus lactis D4, exhibited a significant reduction in fibrosis (7.11 ± 1.14%, p = 0.001), lower than both the Sham and P1 groups. Statistical analysis showed significant differences in fibrosis levels among the groups (p = 0.001). Conclusion : Lactococcuslactis D4 significantly reduced renal fibrosis in the UUO rat model, indicating its potential as a therapeutic agent for inhibiting renal fibrosis. Further studies are required to explore the molecular mechanisms and clinical applications of Lactococcus lactis D4 in renal fibrosis and chronic kidney disease.

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