IN VITRO STUDY OF Curcuma sumatrana Miq. GROWTH ON MODIFIED MURASHIGE AND SKOOG MEDIA AND THE ADDITION OF GLUTAMINE

Stia Prihatiningsih, Prihatiningsih Idris (2024) IN VITRO STUDY OF Curcuma sumatrana Miq. GROWTH ON MODIFIED MURASHIGE AND SKOOG MEDIA AND THE ADDITION OF GLUTAMINE. Diploma thesis, Universitas Andalas.

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Abstract

Curcuma sumatrana Miq. (Zingiberaceae) is an endemic plant species from West Sumatera. This species has a small population size and limited distribution area in the natural habitat, so in vitro propagation for conservation efforts is needed. Basal media composition and supplementation of amino acid are the important keys for successful of plant propagation using in vitro technique. In this research, modification of macronutrient on Murashige-Skoog (MS) media and supplementation of glutamine (amino acid source) were tested. Interaction between these treatments was observed to improve the growth of shoot explants. This research was carried out experimentally using a completely randomized design in factorial, i.e., modification of MS macronutrients (full-, half-, and quarter-strengths) and supplementation of glutamine (0, 50, and 100 mg.L-1). The results show that MS full had a significant effect on the increment of plantlet height (6.73 and 10.34% higher than MS half, and MS quarter, respectively). Supplementation of 50 mg.L-1 glutamine into MS media tend to had significant effect on the first day of root emergence, length of the longest roots, and chlorophyll content of plantlet. MS media supplemented with 50 mg.L-1 glutamine are tend to had significant effect on the increment of plant height, first day of root emergence, chlorophyll content (MS full), and the length of the longest root (MS half). In addition, supplementation of basal media with 50 mg.L-1 glutamine improve shoot multiplication and growth.

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