ISOLASI SENYAWA FENOLIK DARI AKAR TAPAK LIMAN (Elephantopus scaber Linn.) DAN UJI POTENSI ANTIOKSIDANNYA

AMELIA, ERISKA (2015) ISOLASI SENYAWA FENOLIK DARI AKAR TAPAK LIMAN (Elephantopus scaber Linn.) DAN UJI POTENSI ANTIOKSIDANNYA. Diploma thesis, Universitas Andalas.

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Abstract

Isolation and antioxidant activity of phenolic compound from Elephantopus scaber Linn and ntotal phenolic content of different extract (hexane, dichloromethane, ethyl acetate, and methanol)of Elephantopus scaber Linn. extracted concecutively have been carried out. DPPH radical scavenging assay used to measured antioxidant activity and determination of total phenolic content measured by Folin-ciocalteau method. Methanol extract (most polar fraction) showed highest antioxidant activity and contain rich phenolic content than other extracts. Phenolic compunds were isolated from methanol extract by using chromatography method with Amberlite XAD-4 resin and yielded two amorphous yellow solid compounds, AESM-1 and AESM-2. Antioxidant activity of these compounds in terms of effective concentration which scavenges 50% radical (IC ) are 211.731±0.366 µg/ml and 225.575±0.219 µg/ml, respectively. Keywords: Elephantopus scaber Linn., antioxidant, DPPH, Folin-Ciocalteau

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