PERBANDINGAN METODE DPPH DAN FRAP MODIFIKASI PADA PENENTUAN KANDUNGAN ANTIOKSIDAN TOTAL DALAM SAMPEL BENGKUANG,BIT DAN KENTANG

Nanda, Siti Hamzaini (2016) PERBANDINGAN METODE DPPH DAN FRAP MODIFIKASI PADA PENENTUAN KANDUNGAN ANTIOKSIDAN TOTAL DALAM SAMPEL BENGKUANG,BIT DAN KENTANG. Diploma thesis, Universitas Andalas.

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Abstract

Stud y of comparison me thod s on determination of total antioxidant content using DPPH and mo dif ied FRAP methods on bengkoan g, beet and pota to had been done.This study proved that both of methods can be used to determine of total antioxidant content on bengkoang, beet and potato that calculated as ascorbic acid using validation .Total antioxidant content of bengkoang, potato and beet used DPPH and modified FRAP methods respectively (0,765 0 ; 0,6491 ; 0,0 999) mg/g and (0,2547 ; 0,0963 ; 1,0523) mg/g. Statistical parameters f or validation include are, precision, correlation coeff icient LoD and LoQ, % recovery and Horwitz ratio. Statistical parameters obtained for DPPH and modified FRAP methods of beet, potato and bengkoang respectively SDR (2,39 ; 0,42 ; 2.3 1)% and (0,63 ; 2,12 ;1,80 )%, r = -0,9 905 and 0,9896 , LoD 1,41 mg/L and 1,46 mg/L, LoQ 4,71 mg/L an d 4, 89 mg/L , % recover y (110,3 7 ; 106,71 ; 92,43 ) % and (108, 41 ; 107,71 ; 96, 523) % , HORRAT ratio (1,58; 0,29 ; 2,00) and (0,39 ; 1,9 2 ; 1,41). For analysis of comparison method by F-test and t-test on potato, beet and bengkoang showed same precision with different average significantly for both of methods.

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