Potential of Palm Oil Mill Effluent in Producing Biohydrogen Using Anaerobic Microbial

Asyifa, Ul Husna (2023) Potential of Palm Oil Mill Effluent in Producing Biohydrogen Using Anaerobic Microbial. Diploma thesis, Universitas Andalas.

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Abstract

Research on "Potential of Palm Oil Mill Effluent in Producing Biohydrogen Using Anaerobic Microbial " was conducted from March to May 2023 at the Microbiology Laboratory and Baso Veterinary Center. This study aims to determine the production of Biohydrogen that can be generated from the fermentation that utilizes the metabolic activity of microbes in degrading substrates in the form of Palm Oil Mill Effluent (POME). The treatment is the addition of NaOH 1%, 1.5%, and 2%, addition of H3PO4 2.5%, 5% and 7.5%, addition of Molasses 2%, 4% and 6%. The results showed that biohydrogen production reached 5.817 ml/L, which was obtained with the addition of H3PO4 2.5%, with the addition of NaOH 1.5% showed that biohydrogen production reached 3.717 ml/L and the addition of molasses 6% showed that biohydrogen production reached 5.117 ml/L, meanwhile without treatment is reached 3.050 ml/L. In this study there was also analyze in wastewater loads such as BOD, COD, pH, sugar level and temperature which indicated the role of microorganisms in fermentation. The results of isolation and identification found three types of bacterial isolates from fermentation, namely Bacillus sp.1, Bacillus sp.2 and Proteushspp. Keywords: Biohydrogen, Palm Oil Mill Effluent, Renewable Energy, Microorganism

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