Isolation and Heavy Metal Resistant Test of Biofilm Forming Bacteria in Bungus Ocean Fishing Port (PPS), Padang City.

Fanny, Zulkhairiah (2023) Isolation and Heavy Metal Resistant Test of Biofilm Forming Bacteria in Bungus Ocean Fishing Port (PPS), Padang City. Diploma thesis, Universitas Andalas.

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Abstract

Water pollution by heavy metals is mostly caused by high community activity in the Bungus Ocean Fishery Port (PPS) area, Padang City. Heavy metals are very dangerous because they can accumulate in living things and disrupt the balance of the environmental ecosystem. Research on the Isolation and Resistant Test of Biofilm-forming Bacteria from Bungus Samudra Fishery Port (PPS), Padang City has been conducted from April to October 2022 at the Microbiology Research Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences and Basic and Central Laboratory, Andalas University . The method used in this research is the experimental method which include isolation of bacteria, heavy metal resistant test, biofilm formation test, macroscopic and microscopic observation and measurement of growth profile of isolate. This study aims to find biofilm-forming bacteria that are resistant to heavy metals at a concentration of 400 ppm and to determine the growth profile of bacterial isolates. The results showed that 9 biofilm-forming isolates were found that were resistant to 400 ppm heavy metal, namely 1 Cd-resistant bacterial isolate, 1 Cr-resistant bacterial isolate, 4 Cu-resistant bacterial isolates and 3 Pb-resistant bacterial isolates and the growth of the isolates were different from each other which was influenced by by a resistance mechanism to the specific metal used.

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