PERBANDINGAN METODE DPPH dan Fenantrolin pada PENENTUAN ANTIOKSIDAN DALAM SAMPEL BAYAM (Amaranthus hybridus L.), KANGKUNG (Ipomoea reptans), KATUK (Sauropus adrogynus (L)), dan MANGKOKAN (Poliscias scutellaria) SECARA SPEKTROFOTOMETRI

fitratul, ilahi (2016) PERBANDINGAN METODE DPPH dan Fenantrolin pada PENENTUAN ANTIOKSIDAN DALAM SAMPEL BAYAM (Amaranthus hybridus L.), KANGKUNG (Ipomoea reptans), KATUK (Sauropus adrogynus (L)), dan MANGKOKAN (Poliscias scutellaria) SECARA SPEKTROFOTOMETRI. Diploma thesis, universitas andalas.

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Abstract

ABSTRAK COMPARISON of METHODS and FENANTROLIN DPPH ANTIOXIDANT DETERMINATION of TOTAL in SAMPLES SPINACH (Amaranthushybridus L.), WATER SPINACH (Ipomoea reptans), KATUK (Sauropusadrogynus (L)), andMANGKOKAN (PolisciasScutellaria) bySPECTROPHOTOMETRY Fitratulilahi (Bp: 1210412038) Yefrida, M.Si * Dr. Refilda ** * I supervisor II**supervising Research on the comparison of DPPH and Fenantrolin on the determination of the content of antioxidants in a sample of spinach (Amaranthushybridus L.), water spinach (Ipomoea reptans), katuk (Sauropusadrogynus (l)), and mangkokan (PolisciasScutellaria) has been performed. Statistical parameters used for the validation methods are: linearity, SDR, LoD, LoQ and percent recovery. Comparison of two methods used F-test and t-test. Total antioxidant content in samples of spinach, kale, katuk, and mangkokan with DPPH, respectively for (1.5245; 1.4181; 4.6573; and 0.8898) mg / g DW while testing method consecutive Fenantrolin by (7.0307; 13.7480; 44.8386; and 6.8236) mg / g DW. Both methods have the r value of 0.9966 and 0.9955. LOD value of each method (0.0054 and 0.0551) mmol / L, while the value of each method LOQ (0.0180 and 0.1837) mmol / L. Based on the value of the SDR and recovery both valid method for the determination of total antioxidant content in a sample of spinach, kale, katuk, and mangkokan. F test showed that both methods have a significantly different accuracy with a more thorough method is DPPH seen from the SDRnya. The t-test showed that both the results obtained have on average significantly different. Keywords: DPPH, Fenantrolin method, and antioxidant

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