Isolasi dan Karakterisasi Struktur Kumarin Dari Tumbuhan Subang-Subang (Spilanthes paniculata Wall. Ex DC)

SUCI, HEPY ASTUTI (2016) Isolasi dan Karakterisasi Struktur Kumarin Dari Tumbuhan Subang-Subang (Spilanthes paniculata Wall. Ex DC). Diploma thesis, Universitas Andalas.

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Abstract

Isolation of coumarin compound from ethyl acetate extract of Subang-Subang (Spilanthes paniculata Wall. Ex. DC.) was successfully established by column and preparative thin layer chomatography. Ethyl acetate extract was separated by column chromatography using silica gel as stationary phase. n-Hexane, ethyl acetate and methanol were used as mobile phase and then purified by preparative thin layer chromatography with n-Hexane : ethyl acetate (1:1) as the eluent. Isolated compound was obtained in the form of 11 mg white solid. Purity test of isolated compound with TLC (Thin Layer Chromatography) by using 10% NaOH has shown the blue colour fluorecence and UV spectrophotometer have shown the absorption peaks at wavelength 260 nm and 357 nm. Isolated compound was characterized by FT-IR spectrophotometer has shown the -OH stretching at the wave numbers 3435 cm-1. Their absorption bands at wave numbers 2917 cm-1 which indicate the C-H stretching, the wave numbers of 1654 cm-1 indicate stretching C=O and absorption bands at wave numbers 1535 cm-1 has shown C=C stretching aromatic. Absorption bands at wave numbers 1462 cm-1 indicate the presence of C-H bending. Absorption bands at wave numbers 1241 cm-1 and 1165 cm-1 indicate the presence of ester groups. Based on the results of characterization, isolated compound was classified into scopoletine coumarin compounds. Bioactivity test as antibacterial of ethyl acetate extract and scopoletine coumarin compounds by disk diffussion method have shown that ethyl acetate extract and scopoletine coumarin compounds are inactive against Neisseria sp bacteria.

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