Exploration and Potential of Bacteria Isolates from Ngalau Basurek, Sijunjung, for Heavy Metal Immobilization Through Microbial Induced Carbonate Precipitation (MICP)

Luvena, Nesha Fadhilah (2025) Exploration and Potential of Bacteria Isolates from Ngalau Basurek, Sijunjung, for Heavy Metal Immobilization Through Microbial Induced Carbonate Precipitation (MICP). S1 thesis, Universitas Andalas.

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Abstract

Heavy metal contamination particularly by nickel (Ni) and copper (Cu), poses environmental and health risks, especially in rapid industrial countries. Microbially Induced Calcium Carbonate Precipitation (MICP) has emerged as a promising method for bioremediation and biocementation. Ngalau Basurek have unique microbial communities and geochemical conditions, offer great potential as sources of ureolytic bacteria. This study investigates the potential of indigenous cave bacteria to facilitate MICP under heavy metal stress, supporting eco-friendly bioremediation, and soil stabilization from Ngalau Basurek Cave. This utilized a survey based to isolate and evaluate ureolytic bacteria. Through laboratory analyses including urease activity assays, heavy metal tolerance tests, and precipitation evaluations. The findings identified bacterial genus for application in MICP process. The results showed that four ureolytic isolates were obtained, GUA-UB2 exhibited the highest specific urease activity 5.3 hydrolyzed·min⁻¹·OD⁻¹ (40 h) under Ni stress and 1.807 urea hydrolyzed·min⁻¹·OD⁻¹ (1 2 h) under Cu stress. It also demonstrated stable carbonate precipitation over 48 hours, with optimal precipitation observed at 1.6 g/L Ni and 1.2 g/L Cu. Morphological and biochemical characterization suggested that GUA-UB2 is closely related to the genus Proteus, highlighting its strong potential for use in heavy metal bioremediation via MICP.

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