PENGARUHFRAKSI MINYAK ATSIRI UMBI RUMPUT TEKI (Cyperus rotundusL.) TERHADAP APOPTOSIS SERTAEKSPRESIPROTEIN BAX DAN BCL-2 PADA CELL LINE KANKER SERVIKS HELA

dr, susanti (2017) PENGARUHFRAKSI MINYAK ATSIRI UMBI RUMPUT TEKI (Cyperus rotundusL.) TERHADAP APOPTOSIS SERTAEKSPRESIPROTEIN BAX DAN BCL-2 PADA CELL LINE KANKER SERVIKS HELA. Doctoral thesis, Universitas Andalas.

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Abstract

ABSTRAK AKTIVITAS SITOTOKSIK, APOPTOSIS, EKSPRESI PROTEIN BAX DAN BCL-2 FRAKSI MINYAK ATSIRI UMBI RUMPUT TEKI (Cyperus rotundusL.) PADA CELL LINE KANKER SERVIKS HELA Salah satu bahan alam yang potensial untuk dikembangkan sebagai antikanker adalah rumput teki(Cyperus rotundus L.). Penelitian ini bertujuan untuk mengkaji pengaruh fraksi minyak atsiri umbi rumput teki terhadap apoptosis, protein Bcl-2 (anti apoptosis) dan Bax (pro apoptosis). Metode yang dilakukan dalam penelitian ini adalah uji sitotoksik, uji apoptosis dan uji ekspresi protein Bax dan Bcl-2 pada cell line kanker serviks HeLa. Uji sitotoksik minyak atsiri rumput teki beserta 4 fraksinya dilakukan dengan metode MTT assay, yang diukur dengan inhibitory concentration (IC50) menggunakan analisis regresi probit.Uji apoptosis menggunakan flowsitometri dan pengecatan Annexin V/ Pi serta uji ekspresi protein Bax dan Bcl-2menggunakan metode immunositokimia. Dari penelitian ini didapatkanIC50fraksi 1 8,307 + 0,186 μg/ml, fraksi 2 21,377 + 9,543 μg/ml, fraksi 3 1.707,521+1.048,319μg/ml dan fraksi 4 4.398,836+3.476,323μg/ml. Persentase sel apoptosis untuk kontrol 2,38, dosis ½ IC50 4,73, dosis IC50 11,15. Persentase sel yang mengekspresikan protein Bax untuk kontrol 20,725, dosis ½ IC50 35,191, dan dosis IC50 sebsesar 45,945. Persentase sel yang mengekspresikan protein Bcl-2 utk kontrol 74,467, dosis ½ IC50 67,012 dan dosis IC50 55,553, dengan nilai p <0,05. Kesimpulan pada penelitian ini adalah fraksi 1 memiliki inhibitory concentration 50% (IC50) paling kecil terhadap cell linekanker serviks HeLa, Fraksi minyak atsiri umbi rumput teki berpengaruh terhadap peningkatan apoptosis, peningkatan ekspresi protein Bax dan penurunan ekspresi protein Bcl-2 pada cell linekanker serviks HeLa. Kata Kunci:Cyperus rotundus L., Rumput Teki, Kanker serviks, Bax, Bcl-2 ABSTRACT THE INFLUENCES OF PURPLE NUTSEDGE (Cyperus rotundus L.) TUBER ESSENTIAL OIL FRACTION TOAPOPTOSIS AND EXPRESSION OF BAX AND BCL-2 PROTEIN ONTHE HELA CERVICAL CANCER CELL LINE One of the potential natural substance to develop as anticancer agent is the purple nutsedge (Cyperus rotundus L.). This study aims to explore the effect of essential oil fraction of purple nutsedge to apoptosis, the Bcl-2 and Bax Protein. The method used in this research is cytotoxic, apoptosis and immunocytochemistry test. The cytotoxic test of purple nutsedge essential oil with 4 fractions was done by MTT assay method, measured by inhibitoryconcentration (IC50) using probit regression analysis. Apoptotic test using flowsitometry and Annexin V / Pi staining and expression test of Bax and Bcl-2 proteins using immunocytochemistry test. The resulsts of this research areIC50of fraction 1 is 8.307 + 0.186μg / ml, fraction 2 is 21.377+9.543μg / ml, fraction 3 is 1,707.521+1,048.319μg/ml and fraction 4 is4,398.836+3,476.323μg/ml. The percentage of apoptosis for control is 2,38, dose ½ IC50is 4,73, and dosage of IC50is 11,15. The percentage of cells expressing Bax protein for control is 20,725, dose ½ IC50is 35,191, and dose IC50is 45,945.The percentage of cell expressing Bcl-2 protein for control is 74,467, dose ½ IC50is 67,012 and doseIC50is 55,553, with p value<0,05. The conclusion of thisresearch arefraction 1 have smallest IC50 among others, the fraction of purple nutsedge tuber essential oil has influencesto the increase of apoptosis, the increase of Bax protein expression and the decrease of Bcl-2 protein expression on HeLa cervical cancer cell line. Keywords: Cyperus rotundus L., Purple Nutsedge, Cervical Cancer, Bax, Bcl-2

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