ISOLATION AND CHARACTERIZATION OF PROBIOTIC BACTERIA FROM LAPHET (MYANMAR’S TRADITIONAL FERMENTED TEA LEAVES): AND ANALYSIS OF IL-6, TNF-α AND IL1-β GENES EXPRESSION IN DSS INDUCED ANIMAL MODEL COLITIS

OO, THET PAING (2023) ISOLATION AND CHARACTERIZATION OF PROBIOTIC BACTERIA FROM LAPHET (MYANMAR’S TRADITIONAL FERMENTED TEA LEAVES): AND ANALYSIS OF IL-6, TNF-α AND IL1-β GENES EXPRESSION IN DSS INDUCED ANIMAL MODEL COLITIS. Masters thesis, Andalas University.

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Abstract

Laphet (Myanmar’s traditional fermented tea leaves) is one of the most consumed daily snacks throughout Myanmar and it contains Latic Acid Bacteria (LAB) which plays an important role in probiotics. Probiotics are live microbial food constituents that affect the microflora and provide health benefits to the host when consumed in sufficient quantities. This study used the catalase assay and the type of fermentation to assess the features and biochemical properties of LAB. Inflammatory bowel disease (IDB), a chronic relapsing and remitting sickness that includes Crohn's disease (CD) and Ulcerative colitis (UC), has an unknown origin. CD and UC are unidentified causes of chronic inflammatory gastrointestinal diseases. Laphet is one of Myanmar traditional food derived from tea leaves (Camellia sinensis) and it has abundance of lactic acid bacteria, antioxidant properties, catechin, and probiotic properties. Tumor necrosis factor-α (TNF)-α, interleukin-1 beta (IL)-1β and interlukin-6 (IL-6) genes play a pivotal role in IDB expression and these genes was measured using quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR). The present study aims to investigate the anti-inflammatory effect and expression in DSS induce animal model treated with Laphet extract. According to the data presented in this sample, the p values for IL-6, TNF- α, and IL-1β are 0.500, 0.68, and 0.08, respectively. These p values imply that the observed differences in gene expression for IL-6, TNF- α, and IL-1β between the Laphet's extract-treated group and the control group are not statistically significant.

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