PENGARUH PEMBERIAN BAKTERI PENGHASIL SIDEROFOR (Bacillus RK 12) DAN BIOCHAR SEKAM PADI TERHADAP KADAR Fe (BESI) PADA TANAH SAWAH VULKANIS

Catherin, Anggi Tiara (2020) PENGARUH PEMBERIAN BAKTERI PENGHASIL SIDEROFOR (Bacillus RK 12) DAN BIOCHAR SEKAM PADI TERHADAP KADAR Fe (BESI) PADA TANAH SAWAH VULKANIS. Diploma thesis, Universitas Andalas.

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Abstract

Besi (Fe) merupakan unsur keempat yang#paling#banyak ditemukan pada bagian lithosfer bumi namun apabila kadar besi lebih dari 300 ppm dalam tanah maka berpotensi meracun tanaman padi. Penelitian mengenai Pengaruh Pemberian Bakteri Penghasil Siderofor (Bacillus RK 12) Dan Biochar Sekam Padi Terhadap Kadar Fe (Besi) Pada Tanah Sawah Vulkanis telah dilakukan di Rumah)Kaca Fakultas Pertanian(Universitas)Andalas dan analisis tanah di Laboratorium Jurusan Tanah Fakultas Pertanian Universitas Andalas dari bulan Oktober 2019 – Maret 2020. Tujuan dari penelitian ini yaitu (1) Mendapatkan interaksi dari bakteri penghasil siderofor (Bacillus RK 12) dengan media tumbuhnya biochar sekam padi dalam mengatasi keracunan Fe (besi) pada tanah sawah vulkanis, (2) Mendapatkan takaran dosis isolat bakteri penghasil siderofor (Bacillus RK 12) dalam mengatasi keracunan Fe (besi) pada tanah sawah vulkanis serta (3) Melihat pengaruh biochar sekam padi sebagai media tumbuh bakteri penghasil siderofor (Bacillus RK 12) dalam mengurangi keracunan Fe (besi) pada tanah sawah vulkanis. Penelian ini dilakukan secara eksperimental menggunakan rancangan faktorial dengan faktor A (Bakteri penghasil siderofor 0 ml, 10 ml dan 20 ml) dan faktor B (Biochar sekam padi 0 g dan 25 g) dan diulang sebanyak 3 kali sehingga terdapat 18 unit satuan percobaan yang ditempatkan menurut Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan pemberian bakteri penghasil siderofor (Bacillus RK 12) dengan media tumbuhnya biochar sekam padi belum memperlihatkan interaksi terhadap penurunan kadar Fe (Besi) pada tanah sawah vulkanis. Takaran dosis bakteri penghasil siderofor (Bacillus RK 12) 10 ml dapat menurunkan Fe3+ dari 2096 ppm menjadi 2064 ppm dan Fe2+ dari 1409 ppm menjadi 1376 ppm tetapi pada takaran dosis 20 ml belum terjadi penurunan kadar Fe3+ dan Fe2+ pada tanah sawah vulkanis serta pemberian biochar sekam padi 25 g belum mampu menjadi media pertumbuhan bakteri penghasil siderofor (Bacillus RK 12) karena belum adanya penurunan kadar Fe pada tanah sawah vulkanis. Kata kunci : bakteri penghasil siderofor, biochar sekam padi, keracunan Fe, tanah sawah Iron (Fe) is the fourth element the most commonly found in the earth's lithosphere, but if the iron content is more than 300 ppm in the soil, it has the potential to poison rice plants. A research on the Effect of Siderophore Producing Bacteria (Bacillus RK 12) And Rice Husk Biochar on Iron (Fe) Levels in Volcanic Rice Fields was carried out at the Glass House, Faculty of Agriculture, Andalas University and soil analysis at the Laboratory of the Department of Soil Science, Faculty of Agriculture, Andalas University from October 2019 to Maret 2020. The objectives of this study were (1) to obtain the interaction between siderophore producing bacteria (Bacillus RK 12) and rice husk biochar growing media in overcoming Iron (Fe) poisoning in volcanic rice soil, (2) to obtain the dosage of siderophore producing bacterial isolates (Bacillus RK 12) in overcoming Iron (Fe) poisoning in volcanic rice soil and (3) to observe the effect of rice husk biochar as a growing medium for siderophore producing bacteria (Bacillus RK 12) in reducing Iron (Fe) poisoning in volcanic rice soil. This research was conducted experimentally using a factorial design with the first factor was Bacteria (A) having 3 levels (0 ml, 10 ml and 20 ml) and the second factor was Biochar (B) having 2 levels (0 g, 25 g) with 3 replications. There were totally 18 experimental units which were allocated according to a completely randomized design (CRD). The result showed that there was no interaction between siderophore producing bacteria (Bacillus RK 12) and biochar in reducing Fe levels in volcanic paddy soil. The dose of siderophore producing bacteria (Bacillus RK 12) 10 ml could reduce Fe3+ from 2096 ppm to 2064 ppm and Fe2+ from 1409 ppm to 1376 ppm however application of siderophore producing bacteria (Bacillus RK 12) did not decrease Fe3+ and Fe2+ levels in volcanic paddy soil. Application of 25 g of rice husk biochar was not able to be a good growth medium for siderophore producing bacteria (Bacillus RK 12) because there was no reduction in Fe content in volcanic paddy soil. Keywords: siderophore producing bacteria, rice husk biochar, Fe poisoning, paddy soil

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