Pengaruh Kadar Nitrat dan Fosfat Dalam Medium Pertumbuhan Mikroalga Scenedesmus dimorphus untuk Meningkatkan Lipid Serta Asam Lemak ω-6 dan ω-9

utami putri, rahmi (2020) Pengaruh Kadar Nitrat dan Fosfat Dalam Medium Pertumbuhan Mikroalga Scenedesmus dimorphus untuk Meningkatkan Lipid Serta Asam Lemak ω-6 dan ω-9. Diploma thesis, universitas andalas.

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Abstract

In recent years, microalgae have developed rapidly in the development of the health sector. Microalgae grown under normal conditions will contain little lipids and omega 6 and 9. fatty acids. The content of lipids and fatty acids in microalgae is influenced by nitrogen and phosphate nutrients. Therefore treated with variations in the concentration of NaNO3 in the medium and variations in the concentration of KH2PO4 in the medium to increase lipid content. Scenedesmus dimorphus microalgae growth medium uses Bold Basal Medium (BBM) by adding variations in concentration in the medium, including variations in nitrate (20 g / L, 22.5 g / L, 27.5 g / L) and phosphate variation (12.5 g / L, 15 g / L, 20 g / L). Lipids from dried microalgae biomass were extracted using the bligh and dyer method, after the lipids were obtained then transesterified. Furthermore, the results of transesterification were analyzed with Gas Chromatography-Mass Spectra (GC-MS). The results showed that from variations in the concentration of NaNO3 nutrients and KH2PO4 variations carried out, it was obtained a lot of lipid results in each omega at low concentrations of NaNO3 20 g / L for the variation of nitrate nutrient concentrations and KH2PO4 12.5 g / L for variations in concentration phosphate nutrients. Omega-6 & 9 fatty acids contained in variations of nitric and phosphate nutrients are oleic acid, 9-oktadekenoic acid, 6-oktadekenoic acid, 9,12-octadecadienoic acid (linoleic), and 9,12,15-octadecatrienoic acid. Keywords: NaNO3, KH2PO4, Omega 6 dan 9, Scenedesmus dimorphus, Gas Chromatography-Spectra Mass (GC-MS)

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