Pengaruh Tinokrisposid Rich Fraction terhadap Differensiasi Sel Adiposit 3T3-L1

Annisa, Fauzana (2016) Pengaruh Tinokrisposid Rich Fraction terhadap Differensiasi Sel Adiposit 3T3-L1. Masters thesis, Universitas Andalas.

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Abstract

ABSTRACT Tinocrisposide is a isolated furanoditerpen glycoside from Tinospora crispa, that has antidiabetic effect in vivo. Differentiated adipocyte cells are sensitive to insulin because of increasing on insulin’s receptor expression on cell adipocyte membrane. Adipocyte cell differentiation activity of tinocrisposide in interval concentrations of 50; 25; 12.5; 6.25µg/mL has been investigated on 3T3-L1 cell line using insulin of 1µg/mL as a positive and DMEM as a negative control group. The effect of tinocrisposide was quantified with Oil-Red O staining methode by measuring absorbance of lipid solution in 100% isopropanol at wave length (λ) 520 nm. Tinocrisposide in the concentrations of 50; 25; 12.5; 6.25µg/mL, insulin of 1µg/mL, and DMEM groups showed absorbance value of 0.7669; 0.7253; 0.6563; 0.6481; 0.954, and 0.2653 respectively. It was found there were a significant statistically difference of lipid droplets accumulation amongs all groups (p<0.05) and tinocrisposide concentration of 50µg/mL stimulates the highest lipid droplets accumulation on 3T3-L1 adipocyte cells. It can be concluded that tinocrisposide had insulin’s mimicing action through its ability to stimulates adipocyte cell differentiation, but it had lower activity than insulin. Keywords: 3T3-L1 adipocyte cell differentiation, tinocrisposide, Tinospora crispa

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