In Vitro Shoot Multiplication of Curcuma Sumatrana Miq. Using Combination of Thidiazuron and 6-Benzylaminopurine on Modified Murashige and Skoog Media

Daraqutni, Mimma Latifah (2025) In Vitro Shoot Multiplication of Curcuma Sumatrana Miq. Using Combination of Thidiazuron and 6-Benzylaminopurine on Modified Murashige and Skoog Media. S1 thesis, Universitas Andalas.

	Text (Abstrak) Abstrak.pdf - Published Version Download (85kB)
	Text (BAB 1 Pendahuluan) BAB 1 Pendahuluan.pdf - Published Version Download (52kB)
	Text (BAB 5 Penutup) BAB 5 Penutup.pdf - Published Version Download (31kB)
	Text (Daftar Pustaka) Daftar Pustaka.pdf - Published Version Download (96kB)
	Text (skripsi fulltext) skripsi fulltext.pdf - Published Version Restricted to Repository staff only Download (1MB) | Request a copy

Abstract

Curcuma sumatrana Miq., known as “koenih rimbo,” is an endemic and vulnerable species from West Sumatra that requires conservation through in vitro propagation. This study aimed to evaluate the effects of thidiazuron (TDZ) and 6-benzylaminopurine (BAP) on shoot multiplication of C. sumatrana using modified murashige and skoog (MS) media supplemented with 50 mg.L-¹ glutamine. The experiment used a factorial randomized design with 12 combinations of TDZ (0; 0.25; 0.50; and 1 mg.L-¹) and BAP (0; 1; and 2 mg.L-¹), each with five replications. Parameters observed after eight weeks included explant survival, shoot emergence, number and height of shoots, leaf formation, and rooting response. The results showed that TDZ significantly affected shoot height, number of shoots producing leaves, number of leaves, root formation, and root length, while BAP and the interaction between TDZ and BAP showed no significant effect on all parameters. The combination of 0.50 mg.L-¹ TDZ and 2 mg.L-¹ BAP produced the highest average number of shoots (7 shoots per explant). These results indicate that TDZ plays a more dominant role in shoot and root development. Future studies are recommended to use TDZ as a single cytokinin treatment and to apply radial explant cutting by removing the main shoot apex to better assess cytokinin effectiveness on shoot initiation and multiplication.

Actions (login required)

View Item