Isolation and Screening of Potential Thermophilic Bacteria Producing Antibiotics In Cupak and Batu Bajanjang Geothermal Areas, Solok Regency

Enda Tarni, Asih (2025) Isolation and Screening of Potential Thermophilic Bacteria Producing Antibiotics In Cupak and Batu Bajanjang Geothermal Areas, Solok Regency. S1 thesis, Universitas Andalas.

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Abstract

Reseacrh on Isolation and Screening of thermophilic bacteria producing antibiotics from Cupak and Batu Bajanjang Geothermal areas, Solok Regency has been carried out at the Laboratory of Biotechnology, UPT Biota Sumatera, Andalas University since November 2024 until February 2025. Bacteria were Isolated from hot springs at the Cupak and Batu Bajanjang, Solok Regency. This study aims to obtain thermophilic bacteria and obtain characterization of thermophilic bacteria producing antibiotic. This study used purposive sampling method based on gradient temperatures in hot springs which ranged from 45 ℃ - 55℃. Bacterial colonies were inoculated using Nutrient Agar (NA). The result isolation of this study obtained 14 isolates, of which 7 bacterial isolates showed antibiotic producer. The TUA 103 isolate originates from Padang Dama Hot Springs with temperature 54℃, has the highest inhibition zone 8.4 mm against S. aureus and TUA 109 isolate originates from Garara Hot Springs with temperature 52℃, has the highest inhibition zone 6.56 mm against E. coli. TUA 103 has microscopic characteristic such as gram positive properties, basil colony forms, positive endospore staining, positive catalase test, and motilily. TUA 109 has microscopic characteristic such as gram negative properties, diplobacil colony forms, negative endospore staining, positive catalase test and motility.

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