Uji Daya Hambat Ekstrak Biji Petai (Parkia Speciosa Hassk.) Terhadap Bakteri Escherichia coli Dengan Metode Difusi Cakram

Livia, Tiara Astriani (2025) Uji Daya Hambat Ekstrak Biji Petai (Parkia Speciosa Hassk.) Terhadap Bakteri Escherichia coli Dengan Metode Difusi Cakram. S1 thesis, Universitas Andalas.

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Abstract

Escherichia coli is one of the normal flora in the body, especially in the digestive tract. Some strains of this bacterium are pathogenic and can cause infectious diseases. Petai (Parkia speciosa Hassk) has the potential to be a medicinal plant. Phytochemical compounds in petai seeds such as alkaloids, flavonoids, saponins and terpenoids are known to have antibacterial activity. This study aims to determine the inhibition of petai seed extract against Escherichia coli bacteria. Experimental research on disc diffusion method by testing 6 treatment groups, namely extract concentration 25%, 50%, 75%, 100%, positive control (chloramphenicol) and negative control (aquadest) 4 times. The extraction of petai seeds is carried out using methanol solvents. Sterile disc paper containing the treatment substance is placed on a culture of Escherhichia coli bacterial media, the clear zone formed is measured as inhibition. The data obtained was statistically analyzed. The results showed that the average diameter of the barrier zone was 0 mm (25%), 0 mm (50%), 7.25 mm (75%), 8.25 mm (100%), 24 mm (chloramphenicol), and 0 mm (aquadest). The conclusion of this study is petai seed extract (Parkia speciosa Hassk.) has a weak antibacterial effect against Escherichia coli bacteria. There was a significant difference between the concentration of petai seed extract and the growth of Escherchia coli bacteria.

Item Type: Thesis (S1)
Supervisors: dr. Roslaili Rasyid, M. Biomed; Dra. Apt. Yustini Alioes, M.Si
Uncontrolled Keywords: antibakteri; biji petai (Parkia speciosa Hassk.); Escherichia coli;metode difusi cakram
Subjects: R Medicine > R Medicine (General)
Divisions: Fakultas Kedokteran > S1 Kedokteran
Depositing User: S1 Pendidikan Kedokteran
Date Deposited: 19 Jun 2025 03:14
Last Modified: 19 Jun 2025 03:14
URI: http://scholar.unand.ac.id/id/eprint/497621

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