OPTIMASI KULTUR Spirulina platensis UNTUK PRODUKSI SENYAWA ANTIOKSIDAN

DESI NUR, AKBARI (2016) OPTIMASI KULTUR Spirulina platensis UNTUK PRODUKSI SENYAWA ANTIOKSIDAN. Diploma thesis, UNIVERSITAS ANDALAS.

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Abstract

Spirulina platensis is a unicellular microalgae that have high nutritional value such as proteins, carbohydrates, lipids and carotenoids like β-carotene. The microalgae rapidly grow in Bold Bassal Medium modification and high intensity of light. The aims of this research were to optimize the culture growth and determine antioxidant activity of S. platensis. Culture was optimized by adding urea and NaHCO3 into the medium and applying dark : light period (12:12 h/h) under sunlight and fluorescent light (3000 lux). The optimum growth of S.platensis was in 0,3 g/L urea, pH 9 at fluoresense light (3000 lux). Antioxidant acivity of various spirulina extract were studied by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging method. The Spirulina radical scavenging activity of acetone: methanol (1:1) extract was 46,39 %. The acetone: methanol (1:1) extract showed the best antioxidant activity than acetone extract. The β-carotene was determined using HPLC in a silica gel column with dichloromethane, acetonitrile, methanol (20:70:10, v/v/v) as a mobile phase. The acetone ectract displayed the highest sample area and produced 1.32% of β-carotene out of total biomass.

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