Isolasi Jamur Endofit dari Tanaman Betadin (Jatropha multifida L.) dan Uji Aktivitas Antibakteri

Rahmi, Syarifah Masri (2024) Isolasi Jamur Endofit dari Tanaman Betadin (Jatropha multifida L.) dan Uji Aktivitas Antibakteri. Diploma thesis, Universitas Andalas.

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Abstract

Antibacterial resistance is a worldwide health issue, making it challenging to treat certain infectious diseases using conventional antibiotics. This study aims to discover novel antibacterial compounds synthesized by endophytic fungi found in betadin plants (Jatropha multifida L.). The direct planting and pouring methods were employed to isolate endophytic fungi from the roots, stems, leaves, and fruit of betadin plants from Padang, West Sumatra. The isolated endophytic fungi were subsequently cultivated on rice media and extracted using ethyl acetate solvent. The agar diffusion method was employed to screen the antibacterial activity of ethyl acetate extract of endophytic fungi against Staphylococcus aureus, Escherichia coli, and Methicillin-Resistance S. aureus (MRSA). Macroscopic, microscopic, and molecular techniques were employed to identify the fungal isolates with highest antibacterial activity. Fourteen endophytic fungi were identified in this investigation. The ethyl acetate extract of JMB4 and JMD3 fungal isolates exhibited the highest antibacterial activity at a concentration of 5%, as indicated by the antibacterial activity screening results. The growth of S. aureus and MRSA was inhibited by the JMB4 fungal isolate, with inhibitory diameters of 21.79 ± 0.31 mm and 22.55 ± 0.71 mm, respectively. The growth of S. aureus and MRSA was inhibited by the JMD3 fungal isolate, with inhibitory diameters of 23.19 ± 0.58 mm and 23.37 ± 0.77 mm, respectively. The ethyl acetate extract of JMB4 and JMD3 fungal isolates is known to contain alkaloids, flavonoids, steroid, terpenoids, and phenolic compounds, as indicated by the results of the phytochemical examination. According to the analysis, the JMB4 fungus was 100% identical to Fusarium incarnatum, and the JMD3 fungal isolate was 99.09% similar to Fusarium oxysporum. This research has concluded that betadin plants are a potential source of endophytic fungi that produce antibacterial compounds. As a result, further research is necessary to identify secondary metabolite compounds that have the potential to function as antibacterials in the endophytic fungi JMB4 and JMD3. Keywords: Endophytic fungi, Jatropha multifida, antibacterials, Fusarium incarnatum, Fusarium oxysporum

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