DNA BARCODING CHARACTERIZATION OF SUN BEAR (HELARCTOS MALAYANUS, RAFFLES 1821) BASED ON THE COI GENE

M. Ryan, Maulana (2023) DNA BARCODING CHARACTERIZATION OF SUN BEAR (HELARCTOS MALAYANUS, RAFFLES 1821) BASED ON THE COI GENE. Diploma thesis, Universitas Andalas.

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Abstract

Sun bear (Helarctos malayanus) is an endangered species due to illegal trade and habitat loss. Until now, identification of illegal trade confiscated samples has been difficult because the samples are not intact. This study was conducted to create DNA barcodes to assist forensic identification of sun bear samples. Blood samples of sun bears were collected from the Sumatran Tiger Rehabilitation Center Dharmasraya (PR-HSD) totaling three individuals from Riau, Dharmasraya and South Solok. DNA barcode characterization was carried out based on COI gene fragments and compared with 49 sequences of the Ursinae sub-family. Based on the COI gene fragment with a length of 652 bp, three samples of sun bears were found to have different haplotypes. The characteristic DNA sequence that distinguishes Sumatran sun bears from other sun bears is the adenine base at the 652nd sequence. Genetic distance between intraspecies sun bear sequences has a value of 0.78-1.57% and interspecies with a value of 7.83-14.50%. Based on phylogenetic analysis, sun bear samples from Sumatra are clustered into the Sundaland subcluster with low genetic distance (0-0.93%). The results of the study can be used as a reference for identification of sun bears originating from Sumatra. Keywords: DNA barcoding, COI, Helarctos malayanus, Ursinae Phylogenetic

Item Type: Thesis (Diploma)
Primary Supervisor: Dr. Djong Hon Tjong Dr. Rizaldi
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: S1 Biologi Unand
Date Deposited: 29 Dec 2023 00:55
Last Modified: 29 Dec 2023 00:55
URI: http://scholar.unand.ac.id/id/eprint/460217

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