UJI DAYA HAMBAT FILTRAT Lactobacillus plantarum YANG DIISOLASI DARI DADIH TERHADAP PERTUMBUHAN Staphylococcus aureus

Putri, Wulandari (2018) UJI DAYA HAMBAT FILTRAT Lactobacillus plantarum YANG DIISOLASI DARI DADIH TERHADAP PERTUMBUHAN Staphylococcus aureus. Diploma thesis, Universitas Andalas.

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Abstract

ABSTRACT THE EFFECT OF BACTERIOCIN FILTRATE OF Lactobacillus plantarum ISOLATED FROM DADIH ON INHIBITING Staphylococcus aureus GROWTH by Putri Wulandari Lactobacillus plantarum is a lactic acid bacteria producing bacteriocin as an anti-bacterial agent. This research aims to find the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Consentration (MBC) of bacteriocin filtrate from L. plantarum isolated from dadih on inhibiting Staphylococcus aureus growth in vitro. This research was done using dilution technique with completely randomized design. The bacteriocin filtrates of L. plantarum were divided into several concentrations; 100%, 80%, 60%, 40%, 20%, and 0% (control). MIC and MBC were determined by counting the number of growing bacterial colonies in each concentration. The data was statistically analyzed by One-way ANOVA test and Post-Hoc LSD test. The study showed that filtrate of L. plantarum isolated from dadih had an inhibitory effect on the growth of S. aureus. The MIC from bacteriocin filtrate of L. plantarum isolated from dadih was 60% while the MBC cannot be identified. This research concludes that bacteriocin filtrate of L. plantarum has bacteriostatic effect on S. aureus without no bactericidal activity. The anti-bacterial ability of L. plantarum against the growth of S. aureus depends on the concentration of bacteriocin filtrate. Keywords: Antibacterial, bacteriocin, Lactobacillus plantarum, Staphylococcus aureus

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