AKTIVITAS ANTIOKSIDAN DAN KANDUNGAN FENOLIK TOTAL BERBAGAI FRAKSI DARI EKSTRAK METANOL DAUN MELINJO (Gnetun gnemon L.)

Raven, Rahman Rafiqi (2017) AKTIVITAS ANTIOKSIDAN DAN KANDUNGAN FENOLIK TOTAL BERBAGAI FRAKSI DARI EKSTRAK METANOL DAUN MELINJO (Gnetun gnemon L.). Diploma thesis, Universitas Andalas.

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Abstract

Melinjo (Gnetum gnemon L.) contains many active compounds which can be used as antioxidant like flavonoid. Antioxidant activity and total phenolic assay have been studied. Sample was extracted wich methanol by maseration method. Methanol exract was then fractionated with n-hexane, ethyl acetate and buttanol. Antioxidant activity assay was determined by DPPH method. N-hexane, ethyl acetate, n-butanol and methanol/water fraction give IC50 value 219,1 mg/L, 19,50 mg/L, 179,38 mg/L, dan 208.71 mg/L respectively. Related to antioxidant activity, total phenolic assay was determined by Folin-Ciocalteu method. Total phenolic of n-hekxane, etyl acetate, n-butanol and methanol/water fraction are 474,2 mg/L, 1.227,8 mg/L, 730,8 mg/L dan 558,2 mg/L respectively. The highest phenolic total in fraction will give the highest antioxidant activity. Keyword : Gnetum gnemon L., antioxidant, total phenolic contents

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