Dio, Aldo Rano (2017) PENENTUAN MUTASI EKSON 9 DARI GEN PIK3CA PADA PASIEN KANKER PAYUDARA DI SUMATERA BARAT. Diploma thesis, Universitas Andalas.
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Abstract
The mutation of PIK3CA gene altered the activation of phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) in controlling cell proliferation and induced cancer cell formation. Aim of this study is to determine exon 9 mutation of PIK3CA gene in breast cancer by using PCR method and Sanger sequencing method. Genomic DNA was isolated from ten breast carcinomas. Exon 9 of PIK3CA gene was amplified by using specific primer. Furthermore, the purified PCR products were sequenced. The exon 9 of PIK3CA gene sequences was analyzed by Geneious software. The isolated genomics DNA with concentration between 80-140 ng/µL were used to amplify 321 bp PCR products harbor exon 9 of PIK3CA at annealing temperature 59 °C. The exon 9 of PIK3CA gene sequences in tumor samples showed 100% similarity with the NCBI reference sequences of PIK3CA gene. The designed primer successfully amplified region for exon 9 of PIK3CA gene although no mutation found in exon 9 of PIK3CA gene in ten breast cancer patients. Keywords : Breast cancer, Mutation, PIK3CA gene, PCR, Sequencing
Item Type: | Thesis (Diploma) |
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Primary Supervisor: | Dr. rer. nat. Syafrizayanti |
Subjects: | Q Science > QD Chemistry |
Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
Depositing User: | s1 kimia kimia |
Date Deposited: | 25 Jul 2017 10:40 |
Last Modified: | 25 Jul 2017 10:40 |
URI: | http://scholar.unand.ac.id/id/eprint/27014 |
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