OPTIMIZATION OF TEMPERATURE AND pH FOR THE PROTEASE PRODUCTION FROM MANGROVE Sonneratia alba Griff. ENDOPHYTIC FUNGI IN MANDEH AREA, PESISIR SELATAN DISTRICT

Zahrah, Arafah (2023) OPTIMIZATION OF TEMPERATURE AND pH FOR THE PROTEASE PRODUCTION FROM MANGROVE Sonneratia alba Griff. ENDOPHYTIC FUNGI IN MANDEH AREA, PESISIR SELATAN DISTRICT. Diploma thesis, Universitas Andalas.

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Abstract

The research about optimization of temperature and pH for the protease production from mangrove Sonneratia alba endophytic fungi in Mandeh Area, Pesisir Selatan District was conducted on March to June 2023 at Biotechnlogy Laboratory, Andalas University, Padang. This research aims to determine the optimum temperature and pH of endophytic fungal isolates of mangrove plants Sonneratia alba from Mandeh area, Pesisir Selatan District in producing protease and to determine the enzyme production after optimization. The research method used in this study was an experimental and descriptive method. Protease activity was determined using the Takami method. The results of the study showed that optimum temperature for isolates EUA-124 and EUA-126 in producing protease is 34o C and the optimum pH for isolate EUA-124 in producing protease is 7 and the optimum pH for isolate EUA-126 in producing protease is 6. Protease production by isolate EUA-124 increased by 8.621% and protease production by isolate EUA-126 increased by 3.334%.

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