Hubungan ekspresi Ki-67 dan tipe stroma peritumoral dengan varian histopatologik karsinoma sel basal

Silvi, Yelitha (2016) Hubungan ekspresi Ki-67 dan tipe stroma peritumoral dengan varian histopatologik karsinoma sel basal. Masters thesis, Universitas Andalas.

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Abstract

Latar belakang Karsinoma sel basal (KSB) merupakan keganasan kulit tersering pada manusia. Berbagai penelitian faktor risiko dihubungkan dengan perilaku KSB. Faktor-faktor tersebut menyebabkan kerusakan pada tingkat DNA yang mengakibatkan proliferasi sel meningkat. Proliferasi sel merupakan kunci utama progresi tumor yang dapat dinilai dengan ekspresi Ki-67. Studi sebelumnya menunjukkan terdapat hubungan antara penanda proliferasi Ki-67 dengan varian histopatologik KSB, tetapi hal ini masih kontroversi. Jaringan tumor ganas memiliki dua komponen yaitu sel tumor dan stroma. Pada KSB stroma berproliferasi bersama dengan sel tumor. Sepertinya ada hubungan yang saling menguntungkan antara sel tumor dan stromanya. Sel-sel tumor ganas berhubungan dengan stroma peritumoral yang khusus. Tujuan Menilai indeks proliferasi Ki-67 dan tipe stroma peritumoral pada varian histopatologik KSB. Metode Sampel yang digunakan sebanyak 40 terdiri atas 20 sampel KSB agresif dan 20 sampel KSB non agresif. Sampel diwarnai dengan pewarnaan imunohistokima (IHK) Ki-67 dan histokimia (Von gieson dan Alcian blue). Hasil KSB agresif lebih banyak mengekspresikan Ki-67 positif 2 yaitu 78,3% dan memiliki stroma tipe desmoplasia (66,7%), sedangkan KSB non agresif lebih banyak mengekspresikan Ki-67 positif 1 yaitu 88,2% dan memiliki stroma tipe miksoid (70%). Kesimpulan Terdapat hubungan bermakna antara ekspresi Ki-67 dan tipe stroma peritumoral dengan varian histopatologik KSB. Karsinoma sel basal agresif memiliki tipe stroma desmoplasia, sedangkan KSB non agresif miksoid. Secara statistik ditemukan hubungan yang bermakna dengan nilai p < 0,05. Kata kunci Varian histopatogik KSB, KSB agresif, KSB non agresif, Ki-67, indeks proliferasi, tipe stroma peritumoral. ABSTRACT Background Basal cell carcinoma (BCC) is a common skin malignancy in human. Various studies of risk factors associated to the behavior of BCC. These factors cause damage to the DNA level which caused increasing of cell proliferation. Cell proliferation is a key tumor progression which is assessed by Ki-67 expression. Previous studies showed there is a relationship between proliferation marker Ki-67 and histopathological variants BCC, but it is still controversial. Malignant tumor tissue has two components, namely tumor cells and stromal. At BCC stroma proliferate along with the tumor cells. There is a mutually beneficial relationship between tumor cells and stromal. Malignant tumor cells associated with peritumoral stromal special. Objectives Assessing the Ki-67 proliferation index and type of peritumoral stromal on histopathological variants BCC. Method We used 40 samples consisting of 20 samples of aggressive BCC and 20 samples of non aggressive BCC. Each sample was stained by immunohistochemical Ki-67 and histochemistry (Von Gieson and Alcian blue). Result Aggressive BCC expressing Ki-67 positive 2 that is 78,3% and has a desmoplastic type stroma (66,7%), while the more non aggressive BCC expressing Ki-67 positive 1 is 88,2% and has a myxoid stromal type (75%). Conclusion There is a significant relationship between the expression of Ki-67 and type of peritumoral stromal with histopathological variant of BCC. Aggressive BCC has a type of stromal desmoplastic, whereas non aggressive BCC has myxoid. Statistically significant relationship with value of p <0.05. Key words Varian histopathology of BCC, aggressive BCC, non aggressive BCC, Ki-67, proliferation index, type of peritumoral stromal.

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