Isolasi dan Karakterisasi Alkaloid dari Batang Glodokan (Polyathia longifolia) dan Uji Antioksidan

Neli, Asriani (2016) Isolasi dan Karakterisasi Alkaloid dari Batang Glodokan (Polyathia longifolia) dan Uji Antioksidan. Diploma thesis, Universitas Andalas.

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Abstract

Isolation of alkaloid from the stem of Glodokan ( polyalthia iongifolia ) has been done. The stem that have grinded was extracted by maseration using n-hexane, ethyl acetate and methanol continously. Methanol extract was fractination with colomn chromatography using silica gel as stationary phase and methanol, ethyl acetat & hexane as the mobile phase followed by Step Gradient Polarity (SGP). The eigthth fraction of 11 fraction has purified by using preparative thin layer chromatography (TLC) with eluen DCM : aseton ( 9: 1 ) as mobile phase. The isolated compound was white-yellow solid shown the yellow node in dragendorf reagent tested that indicated the isolated compound is alkaloid. Analysis of UV Spectroschopy shown the isolated compound has maximum absorbancy at 202.80 nm wave length. Spectrum of FTIR shown the isolated compound has C-N, C=C, and C-H functional group. Antioxidant activity of methanol extract was tested by DPPH metthod with IC50 of 32.62 mg/L that shown the methanol extract most active as antioxidant and phenolic content was 1882.38 GAE/10 mg dried extract. Keyword: Polyalthia longifolia, Alkaloid, Antioxidant, Fenolic Content, IC50

Item Type: Thesis (Diploma)
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
Depositing User: s1 kimia kimia
Date Deposited: 04 Jan 2017 04:06
Last Modified: 04 Jan 2017 04:06
URI: http://scholar.unand.ac.id/id/eprint/20059

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