REGINA, ANDAYANI (2015) ANALISIS KADAR α-MANGOSTIN DALAM EKSTRAK DAN FRAKSI-FRAKSI KULIT BUAH MANGGIS (Garcinia mangostana L.) SECARA KROMATOGRAFI CAIR KINERJA TINGGI DAN UJI AKTIFITAS SITOTOKSIK PADA SEL KANKER PAYUDARA T47D. Doctoral thesis, UPT. Perpustakaan Unand.
![[img]](http://scholar.unand.ac.id/style/images/fileicons/text.png) |
Text
201504201335th_disertasiku-gabungan.pdf - Published Version Restricted to Repository staff only Download (2MB) |
Abstract
This research aims to develop a cheap, accurate, precise, linear and rapid Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method and validate as per ICH guidelines for the quantitative estimation of α-mangostin in the rind extract and fraction of mangosteen (Garcinia mangostana L.) as well as to determine their cytotoxicity activity against T47D breast cancer cell line and the effect of ethyl acetate pericarp fraction of mangosteen in cell death mechanism of T47D breast cancer cell. The optimized method uses a reverse phase column, Shimadzu ®Shimppack VP – ODS ( 4.6 x 250 mm; 5μ), a mobile phase of 0.1 % v/v H3PO4 in water:acetonitrile (15:85), flow rate of 1ml/min and a detection wavelength of 243,2 nm using a UV detector. The cytotoxic activity against breast cancer cell line T47D was determined as percentage of cell viability by using MTT (Microculture Tetrazolium Assay) colorimetric assay and IC50 (concentration that inhibits cell growth by 50%) were calculated. Apoptosis and necrosis examination was conducted by double staining method using acridine orangepropidium iodide and observed by using fluoresence microscope after 24 hours of incubation. The developed method resulted in α-mangostin eluting at 8.87 min. α- Mangostin exhibited linearity in the range 0.5 – 30 μg/ml, and precise (intra-day variation ≤ 0.10 %, inter-day variation ≤ 2.28 %). The average percentage mean recovery was 94.41-102.01 %, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) was found to be 0.2807 and 0.9357 μg ml respectively. The concentration of α-mangostin in the 70 % ethanol extract, nhexane fraction, ethylacetate fraction and n-butanol fraction were 50.73; 11.12; 98.66; 2.29 % w/w, respectively. This extract and fractions had IC50 of 1.375; 5.879; 0.463; and 51.839 μg/ml against T47D cell line respectively. The ethyl acetate fraction of mangosteen rind caused 43.57 % apotosis effects and 5.60 % necrosis effects. A cheap, accurate, precise, linear and rapid RP-HPLC method was developed and validated for the quantitative estimation of a-mangostin in the rind extract and fraction as per ICH guidelines and hence it can be used for the quality control of crude extract and herbal formulation. The strongest cytotoxic activity was showed by ethyl acetate fraction of fruit rind of Garcinia mangostana L.,
| Item Type: | Thesis (Doctoral) |
|---|---|
| Subjects: | R Medicine > R Medicine (General) R Medicine > RB Pathology R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| Divisions: | Pascasarjana (Disertasi) |
| Depositing User: | Ms Ikmal Fitriyani Alfiah |
| Date Deposited: | 27 Jan 2016 08:28 |
| Last Modified: | 24 Oct 2017 09:44 |
| URI: | http://scholar.unand.ac.id/id/eprint/169 |
Actions (login required)
 |
View Item |
