Fithri, Washliyah Siregar (2023) Potensi dan Karakterisasi Kandidat Bakteri Pendegradasi Lemak Limbah Cair Industri Minyak Kelapa Sawit. Masters thesis, Universitas Andalas.
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Abstract
Lipid-degrading bacteria in Palm Oil Mill Effluent (POME) from processing ponds are capable to lysing lipids through the production of lipase enzymes by lipolytic bacteria or through the acidification pathway by fermentative bacteria. This study aims to obtained the existence of potential bacteria, analyze the in vitro potential of isolates degrading lipids, determine the characteristics of isolates degrading lipids, and analyze the activity of lipid degradation of potential isolates in the wastewater of the palm oil industry. Exploration of potential lipid-degrading bacteria in POME was carried out using a survey method with several stages: detection of bacteria's existence, in vitro testing of potential indexes, characterization, and using experimental method to lipid degradation test and determination of lipase activity. The results showed the existence percentage of several bacteria groups in POME, that is 50-74% lipolytic, 31-90% fermentative, 76-83% proteolytic, and 51-74% cellulolytic bacteria. Selected lipid-degrading isolates have high in vitro potential (lipolytic and fermentative indexes), with E3 had the largest lipolytic index, degradation value (48.72%), lipase activity (0.12 U/ml), and identified as Bacillus cereus CCM 2010. Meanwhile, F2 had the largest fermentative index, degradation value (22.35%), lipase activity (0.01 U/ml), and identified as Bacillus thuringiensis ATCC 10792. Based on this, the selected isolats E3 and F2 had the potential to be used as candidates for biological agents in the process of bioremediation of POME.
| Item Type: | Thesis (Masters) |
|---|---|
| Primary Supervisor: | Dr. phil. nat., Periadnadi |
| Uncontrolled Keywords: | lipid-degrading bacteria, fermentative, lipolytic, POME |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Pascasarjana (S2) |
| Depositing User: | S2 Biologi biologi |
| Date Deposited: | 16 Feb 2023 03:30 |
| Last Modified: | 16 Feb 2023 03:30 |
| URI: | http://scholar.unand.ac.id/id/eprint/123270 |
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