EVALUASI STATUS TAKSONOMI GENUS Geocharis (K.Schum.) Ridl. (ZINGIBERACEAE) DI SUMATERA MENGGUNAKAN KARAKTER MORFOLOGI DAN MOLEKULER (ITS dan matK)

Zulaspita, Witri Zulaspita (2022) EVALUASI STATUS TAKSONOMI GENUS Geocharis (K.Schum.) Ridl. (ZINGIBERACEAE) DI SUMATERA MENGGUNAKAN KARAKTER MORFOLOGI DAN MOLEKULER (ITS dan matK). Masters thesis, Universitas Andalas.

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Abstract

Geocharis is a small genus in the Zingiberaceae family, consisting of 6 species in the world. Geocharis has a high level of flower and fruit variety. Also, the type of Geocharis rubra is a complex species, where the species delimitation in this species is not clear. Thus, it is necessary to evaluate the status by observing the morphological and molecular characters in this genus to strengthen and support the true identity of the species contained in the genus Geocharis. Kinship uses ITS and matK to see groupings between species to aid in classification. The method used in this research is a survey method and observation in the laboratory. The amplified samples were sequenced and analyzed to determine the grouping between species. The morphological characters that contributed to the classification of the genus Geocharis were the shape of the ligule, the direction of growth inflorescences, length of inflorescences, the arrangement of the flower buds, the cross-sectional shape of the ovary, the surface of the ovary, the shape of the fruit and fruit ornaments. Genus Geocharis include ITS sequence length 647- 730 bp, matK 1001-1057 bp, conservative character ITS 511% and matK 954%, informative characters on ITS markers 5% and matK 3%, matK has lower informative characters than ITS marker. The results of the evaluation of the taxonomic status of the Geocharis resulted in 6 types of Geocharis, namely G. macrostemon, G.aurantiaca, G.rubra, G.radicalis, Geocharis sp1 and Geocharis sp2. Two of them Geocharis sp1 (Rimbo Panti) and Geocharis sp2 (Andaleh) were proposed to be new taxa in the genus Geocharis. Keywords : Geocharis, ITS, morphological characters, molecular characters, matK, species delimitation

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