Antimicrobial Potency of Flower and Fruit Extract From Elderberry (Sambucus javanica Blume.) And Its Antioxidant Activities

Putri, Nikita Elsa (2022) Antimicrobial Potency of Flower and Fruit Extract From Elderberry (Sambucus javanica Blume.) And Its Antioxidant Activities. Diploma thesis, Universitas Andalas.

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Abstract

Elderberry (Sambucus javanica Blume.) is a medicinal plant from the Adoxaceae family. S. javanica have health benefit such as antimicrobial, antioxidant, anticancer and anti-inflammatory. Indonesian people have been using S. javanica for a long time as traditional medicine to cure acne, dermatitis and digestive disorders. The role of S. javanica as medicine is supported by its secondary metabolites such as polyphenol, alkaloid and saponin. Previous research about ethanolic extract from the leaf of S. javanica show antimicrobial and antioxidant activity, meanwhile research on flower and fruit from S. javanica in fresh and infusion extract treatment has not been reported. The aim of this research is to determine the antimicrobial and antioxidant activities of flower and fruit extracts from S. javanica. The method of this research is experimental in Nested design with 3 repetitions. The results show that flower and fruit extracts from S. javanica have antimicrobial activity. Based on statistical analysis the effect of each extract treatment against Escherichia coli and Staphylococcus aureus are significantly different, with the largest inhibition zone is fresh fruit extract treatment (11.9 mm) and (13.7 mm) and not significantly different against Candida albicans with the largest inhibition zone is fresh flower extract treatment (6.8 mm). The Minimum Inhibitory Concentration (MIC) of S. javanica against E. coli and S. aureus is 12.5%, meanwhile MIC for C. albicans was not found. Minimum Lethal Concentration (MLC) for all pathogenic microbes was not found. The highest antioxidant activity value of S. javanica was in fresh fruit extract at IC50 (58.74 µg/mL) in strong category.

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