AKTIVITAS ANTIOKSIDAN DAN KANDUNGAN TOTAL FENOLIK EKSTRAK DAN FRAKSI KOLOM ETIL ASETAT DAUN TUMBUHAN Lantana camara L.

Kartika, MZ (2016) AKTIVITAS ANTIOKSIDAN DAN KANDUNGAN TOTAL FENOLIK EKSTRAK DAN FRAKSI KOLOM ETIL ASETAT DAUN TUMBUHAN Lantana camara L. Diploma thesis, Universitas Andalas.

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Abstract

The ethyl acetate extract of Lantana camara L. was subjected to investigate its antioxidant properties by DPPH method and IC50 value was found to be 36.18 mg/L with a total phenolic content was 2419.6 GAE. The concentrated ethyl acetate extract of Lantana camara L. was column chromatographed by separation gradien polarity (SGP) method system using silica gel as the stationary phase then n-hexane, ethyl acetate and methanol as the mobile phases. Seven fractions were obtained, each fractions was subjected to investigate its antioxidant properties and total phenolic content. Thus IC50 value and total phenolic content of each fractions were found to be: Fraction A (132.62 mg/L and 237.8 GAE), Fraction B (113.51 mg/L and 589.4 GAE), Fraction C (85.23 mg/L and 995.5 GAE), Fraction D (81.26 mg/L and 1041 GAE), Fraction E (24.83 mg/L and 3156 GAE), Fraction F (83.50 mg/L and 1037.8 GAE), and Fraksi G (806.71 mg/L). Based on the IC50, extract ethyl acetate and Fraction E are highly active antioxidants, fraction C, D, and F are active antioxidant, fraction A and B moderate antioxidant and fraction G is not aktive antioxidant. Antioxidant activity is influenced by total phenolik with correlation number was 98.14%.

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