ISOLASI DAN PENGUJIAN BIOAKTIFITAS METABOLIT SEKUNDER DARI EKSTRAK KULIT BATANG MATOA (Pometia pinnata, Forst & Forst)

Neni, Trimedona (2016) ISOLASI DAN PENGUJIAN BIOAKTIFITAS METABOLIT SEKUNDER DARI EKSTRAK KULIT BATANG MATOA (Pometia pinnata, Forst & Forst). Doctoral thesis, Universitas Andalas.

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Abstract

Matoa (Pometia pinnata, Forst & Forst), a member of Sapindaceae family which useful for health.The leaves and bark are used in traditionally medicine to treat mouth infections, wounds and burns, abdominal ailments including stomach complaints, diarrhea, dysentery, obstetric and gynaecological complaints. The aim of this research to isolate, structure elucidation and bioactivity evaluation including antioxidant activity and toxicity for brine shrimp Artemia salinafrom extract, the cytotoxicity against P388 murine leukemia cells and antibacterial activity from pure compound.The extraction process was done by maceration technique and purifiedof compounds was carried out by utilising column chromatography.Structure of the isolated compounds determined by analyzing the UV-Vis spectral data, Infra Red, 1H-NMR, 13C-NMR, 2D NMR include HMQC, HMBC, DEPT and COSY) and compared with published spectral data. The results of isolation were obtained four compounds, namely 1) taraxerone as white crystalline with melting point = 241-242oC; 2) stigmasterol as white crystalline with melting point = 164-165oC; 3) taraxerol white crystalline with melting point = 277-278oC and 4) 3,4 dihydroxybenzoic acid as brownish yellow powder with melting point 200-201oC. Antioxidant activity from ethyl acetat, aceton and methanol extract showed active category with IC50 (µg/mL) = 46.04, 11.34 and 14.34 respectively. All the extract of matoa stem bark inhibited toxicity to Artemia salina, but only taraxerol compound showed significant toxicity against P388 with IC50value of 2,76 µg/mL, while stigmasterol, taraxerone and 3,4 dihydroxybenzoic acid did not exhibited toxicity (IC50 = 37,10 µg/mL and IC50> 100 µg/mL, respectively). The result of antibacterial activity showed aceton extract have potentially as antibacterial component because its minimal inhibitory concentration (MIC) have same with control (cefadroxil) with MIC value of 0,1563 µg/mL.

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